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| Registros recuperados: 9.178 | |
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| Polymorphic DNA bands were identified between a near iso-isogenic line of wheat carrying both stem (Sr39) and leaf (Lr35) rust resistance genes and the recurrent line Thatcher (Tc) which lacks these genes. Both resistance genes are located on a translocated chromosomal segment derived from Aegilops speltoides and thus are genetically linked. The primers used to generate polymorphic bands were 3'-anchored inter-simple sequence repeat primers which identified genomic microsatellites with a repeated motif of 3 nucleotides in length. The primers were used singly to amplify genomic segments which were flanked by inversely orientated, closely spaced, identical microsatellite sequences. One of the polymorphic bands, a 900 base pair band, was completely linked to... |
| Tipo: Journal article |
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| Ano: 1999 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581999000100004 |
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| Mahalakshmi,Viswanathan; Ortiz,Rodomiro. |
| Sequencing and analysing of the Arabidopsis thaliana genome, the first plant kingdom genome to be unraveled, will always remain a scientific landmark. International initiatives to sequence rice, the most important cereal in Asia, are underway. However as functional information piles up in Arabidopsis and rice, researchers working in other crops will benefit from this new knowledge and apply it to their studied plants or crop species. The increasing role of public databases of model organisms and bio-informatics in data mining, presents a new opportunity as well as a challenge to researchers to develop more focused molecular tools for gene discovery and deployment. The work presented in here describes how such an approach has benefited sorghum, a rainfed... |
| Tipo: Journal article |
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| Ano: 2001 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000300012 |
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| García-Coronado,Heriberto; Troncoso-Rojas,Rosalba; Tiznado-Hernández,Martín Ernesto; de la Cruz Otero,María del Carmen; Díaz-Camacho,Sylvia Páz; Báez-Flores,María Elena. |
| Background Isothiocyanates (ITCs) are natural products obtained from plants of the Brassicas family. They represent an environmentally friendly alternative for the control of phytopathogenic fungi. However, as it has been observed with synthetic fungicides, the possibility of inducing ITC-resistant strains is a major concern. It is, therefore, essential to understanding the molecular mechanisms of fungal resistance to ITCs. We analyzed a subtractive library containing 180 clones of an Alternaria alternata strain resistant to 2-propenyl ITC (2-pITC). After their sequencing, 141 expressed sequence tags (ESTs) were identified using the BlastX algorithm. The sequence assembly was carried out using CAP3 software; the functional annotation and metabolic pathways... |
| Tipo: Journal article |
Palavras-chave: Blast2GO analysis; Fungal drug tolerance; Isothiocyanates; Natural fungicides. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000400011 |
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| Alves Macedo,Gabriela; Soberón Lozano,Maria Mercedez; Pastore,Gláucia Maria. |
| Short chain citronellyl esters were synthesized by a new microbial lipase from Rhizopus sp strain isolated and lipase produced at UNICAMP, Brazil. Direct esterification and transesterification reactions have been performed to produce citronellyl acetate and butyrate in a free-solvent system and with n-hexane in reaction medium. Reaction mixture for direct esterification and transesterification was carried out at 45ºC in equimolar concentration of substrates: acid or ester and alcohol. Only citronellyl butyrate was synthesized by direct esterification with yields from 95 to 100% after 24 hrs. of reaction time, with or without n-hexane. Citronellyl acetate was synthesized by transesterification with ethyl acetate and cytronellol, with yield of 58% after 48... |
| Tipo: Journal article |
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| Ano: 2003 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000100009 |
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| Assis,Odilio B. G.; Claro,Luis C. |
| The protein lysozyme was deposited onto a permeable support comprising chemically functionalized glass fiber. The main objective of this study was to set a stable organic net with no effect on the medium bed permeability and a preliminary test the activity of this enzyme under immobilized conditions. The film formation is followed by atomic force microscopy (AFM) surface imaging. The effect on the bacteria Escherichia coli was tested using a simple microfiltration column. The filtration results pointed around 75% removal of bacteria in the effluent when compared to the influent concentration. The removal mechanism is assumed as being essentially due biointeraction. The surface polarity characteristics of the formed film were also considered as playing an... |
| Tipo: Journal article |
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| Ano: 2003 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000200010 |
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| Yáñez,Romina; Bastías,Roberto; Higuera,Gastón; Salgado,Oscar; Katharios,Pantelis; Romero,Jaime; Espejo,Romilio; García,Katherine. |
| Background The surveillance of Vibrio parahaemolyticus in the Chilean coast has been mainly performed by multiplex PCR amplification of three different hemolysin genes, which are specie-specific virulence factors. These genes are also employed in the determination of V. parahaemolyticus pathogenic load in seafood and for characterization of pathogenic strains associated to diarrhea cases in human. During environmental surveillance that we performed every summer, we occasionally observed a thermolabile hemolysin (tlh) PCR product of a slightly smaller size than expected, which was coincident with low loads of V. parahaemolyticus in the environment. In order to understand this observation, we probed the specificity of tlh primers for the detection of V.... |
| Tipo: Journal article |
Palavras-chave: Hemolysin; Multiplex PCR; Pathogen surveillance; Virulence factor; Vibrio parahaemolyticus. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600012 |
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| Raih,Mohd Firdaus; Harmin,Sharr Azni; Ahmad,Hafiza Aida; Mat Isa,Mohd Noor; Mahadi,Nor Muhammad; Ibrahim,Abdul Latif; Mohamed,Rahmah. |
| Bioinformatics is a necessary technology and tool, which current molecular biologists cannot do without in light of the genome data explosion and advent of fields such as genomics and proteomics. Yet, even with this in mind, there is still a limited pool of human resource to support and develop bioinformatics particularly in poorer developing nations. The highly multidisciplinary nature of this field can be an obstacle in converting just any molecular biologist into a proficient and capable bioinformaticist. Coupled with the high costs of software, hardware, technical maintenance and systems support; many developing and poor countries may not be able to fully realize their biotech potential either academically or economically due to the inability to fully... |
| Tipo: Journal article |
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| Ano: 2003 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000100003 |
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| Zhao,Yongliang; Dong,Zongming; Li,Tengfei; Huang,Gang. |
| Background: The AdEasy system is a fast-track system for generating recombinant adenoviruses using the efficient homologous recombination machinery between shuttle and adenovirus backbone plasmids in Escherichia coli BJ5183 cells. The key step is homologous recombination in BJ5183 cells, which is driven by RecA activity. However, culture time is stringently limited to reduce the damage to recombinant plasmids by RecA activity. Therefore, rapid identification of recombinant adenoviruses within the limited time-period is critical. Results: We developed a simple negative selection method to identify recombinant adenoviruses using colony PCR, which improves the efficiency of adenovirus recombination screening and packaging. Conclusions: The negative selection... |
| Tipo: Journal article |
Palavras-chave: Adenovirus; AdEasy; Colony PCR; Homologous recombination; RecA. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000100008 |
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| Bhandarkar,Satej V.; Neau,Steven H.. |
| The influences of water activity and solvent hydrophobicity on the kinetics of the lipase-catalyzed enantioselective esterification of flurbiprofen with n-butanol were investigated. The solvent effect was not similar for lipases from Candida rugosa (Crl), Mucor javanicus (Mjl), and porcine pancreas (Ppl). The lipase-catalyzed reaction rates in different solvents across a wide range of water activities revealed that the Ppl-catalyzed reaction exhibited no enantioselectivity and no substantial water activity or solvent dependence. The Mjl-catalyzed reaction proceeded faster, preferring the R-enantiomer reaction over that of its antipode, but had little solvent or water activity dependence. The Crl-catalyzed reactions in n-heptane and n-nonane had similar... |
| Tipo: Journal article |
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| Ano: 2000 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582000000300003 |
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| Zhang,Xiaoyong; Zhou,Jinyan; Fu,Wen; Li,Zhidong; Zhong,Juan; Yang,Jie; Xiao,Liang; Tan,Hong. |
| Response surface methodology (RSM) was used for statistical optimization of jiean-peptide (JAA) production by Bacillus subtilis ZK8 cells adsorbed on wood chips to form a novel fermentation system. The Plackett-Burman design was used in the first step to evaluate the effects of eight factors, including six fermentation medium components and two cell adsorption conditions. Among the variables screened, soybean meal hydrolysate (SMH) and MgSO4·7H2O in the fermentation medium had significanteffects on JAA production. In the second step, the concentrations of SMH and MgSO4·7H2O were further optimized using central composite designs and response surface analysis. The optimized concentration of SMH and MgSO4·7H2O was 24% (v/v) and 0.38% (w/v), respectively,... |
| Tipo: Journal article |
Palavras-chave: Bacillus subtilis ZK8; Cell adsorption; Jiean- peptide; Response surface methodology; Wood chips. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400006 |
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| Schiappacasse,MarÃa Cristina; Palma,Juan; Poirrier,Paola; Ruiz-Filippi,Gonzalo; Chamy,Rolando. |
| In Latin Americ a, the most accepted disposal systems for Municipal Solid Waste (MSW) are landfills, which nowadays have low rates of stabilization. The objective of this study was to develop design criteria for sanitary landfills which lead to a reduction in the stabilization times of MSW, based on experiment results obtain from a pre-pilot scale operation of two sanitary landfills (0.5 Ton), one with recirculation of leachates treated in an anaerobic digester and the other with recirculation of untreated leachates. This was complemented by another pilot scale sanitary landfill (1440 Ton) with recirculation of leaches treated in an anaerobic filter, and additionally by a computer simulation of leachate generation through the water balance of a theoretical... |
| Tipo: Journal article |
Palavras-chave: Hydrological balance; Landfill; Leachate recycle; Municipal solid waste; Stabilization. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300009 |
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| Li,Rui; Wang,Wanjun; Wang,Wenguo; Li,Fosheng; Wang,Qingwei; Xu,Ying; Wang,Shenghua. |
| Background Cysteine proteinase inhibitor (cystatin, CPI) is one of the most important molecules involved in plant development and defense, especially in the regulation of stress responses. However, it is still unclear whether the Jatropha curcas CPI (JcCPI) gene functions in salinity response and tolerance. In this study, the sequence of the JcCPI gene, its expression pattern, and the effects of overexpression in Escherichia coli and Nicotiana benthamiana were examined. The purpose of this study was to evaluate the regulatory role of JcCPI in salinity stress tolerance. Results The CPI gene, designated JcCPI, was cloned from J. curcas; its sequence shared conserved domains with other plant cystatins. Based on a transcription pattern analysis, JcCPI was... |
| Tipo: Journal article |
Palavras-chave: Cysteine proteinase inhibitor; Jatropha curcas; Salinity stress. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000500008 |
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| Cui,Yihong; Zhu,Guangqin; Chen,Qiuju; Wang,Yunfei; Yang,Mingming; Song,Yuxuan; Wang,Jiangang; Cao,Binyun. |
| Complementary DNA (cDNA) is valuable for investigating protein structure and function in the research of life science, but it is difficult to obtain by traditional reverse transcription. In this study, we employed a novel strategy to clone the human leukemia inhibitory factor (hLIF) gene cDNA from genomic DNA directly isolated from the mucous membrane of mouth. The hLIF sequence can be acquired within a few hours by means of amplification of each exon and splicing using overlap-PCR. Thus, the new approach developed in this study is simple, time- and cost-effective, and it is not limited to particular gene expression levels of each tissue. |
| Tipo: Journal article |
Palavras-chave: CDNA cloning; HLIF; Oral mucous membrane; Overlap PCR. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300012 |
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| Benítez-García,Israel; Vanegas-Espinoza,Pablo Emilio; Meléndez-Martínez,Antonio J; Heredia,Francisco J; Paredes-López,Octavio; Del Villar-Martínez,Alma Angélica. |
| Background The properties of natural pigments, such as antioxidants, functional, medical, and nutraceutical, have demonstrated the advantages of these natural compounds over synthetic ones. Some products are accepted only when they are pigmented with natural, food-quality colorants: for example poultry products (manly marigold flower extracts). Carotenoids such as β-carotene, β-criptoxanthin and lutein are very attractive as natural food colorants due to their antioxidant and pro-vitamin activities which provide additional value to the target products. Marigold (Tagetes erecta) is an Asteraceous ornamental plant native to Mexico, and it is also important as a carotenoid source for industrial and medicinal purposes but nowadays its... |
| Tipo: Journal article |
Palavras-chave: 2 4-D; BA Cell development Lutein Marigold. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000300001 |
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| Rywinska,Anita; Rymowicz,Waldemar; Marcinkiewicz,Marta. |
| In the present report, citric acid production from raw glycerol in two fed-batch systems by acetate negative-mutants of Yarrowia lipolytica: Wratislavia 1.31 and Wratislavia AWG7 was compared. In the system, in which the total glycerol concentration was 200 g∙L-1, the substrate was added by pulsed additions, and in the other, in which the total glycerol concentration was 300 g∙L-1, the substrate was added at a constant feeding rate of 1.4 g∙h-1. Despite high citric acid concentrations (155.2 and 157.5 g∙L-1 with Y. lipolytica Wratislavia 1.31 and Y. lipolytica Wratislavia AWG7, respectively) obtained from 300 g∙L-1 of glycerol, the yield of citric acid was similar, i.e. about 0.6 g∙g-1. The... |
| Tipo: Journal article |
Palavras-chave: Citric acid; Fed-batch culture; Yarrowia lipolytica; Raw glycerol. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400009 |
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| Registros recuperados: 9.178 | |
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